PHYTOCHEMICAL-DRIVEN SYNTHESIS OF SILVER NANOPARTICLES USING OCIMUM SANCTUM (TULSI) LEAF EXTRACT
DOI:
https://doi.org/10.66302/api.vol.1.issue.01.05Keywords:
Green synthesis, silver nanoparticles, Ocimum sanctum, plant-mediated synthesis, nanoparticle characterizationAbstract
Green synthesis offers an environmentally sustainable alternative to conventional chemical methods for the production of silver nanoparticles. In the present study, an aqueous leaf extract of Ocimum sanctum was employed as a biological reducing and stabilizing agent for the synthesis of silver nanoparticles. The study was conducted in the Department of Botany, CCR College, Muzaffarnagar, in collaboration with BIO-COSMOS Lab, Meerut, during 2025–2026. The synthesized nanoparticles were characterized using UV–visible spectroscopy and agarose gel electrophoresis. The appearance of a characteristic surface plasmon resonance peak near 420 nm was used to confirm nanoparticle formation, while agarose gel electrophoresis was used to investigate nanoparticle–DNA interactions and their potential effects on DNA integrity. The findings are expected to contribute to a better understanding of the role of O. sanctum phytochemicals in the bioreduction and stabilization of silver nanoparticles and to support the development of safer, plant-mediated nanomaterials.
References
A. V. Narasimha Swamy and R. Srinu Venkat Rao (2021).
Synthesis, characterization and antibacterial activity of
AgNPs mediated by Ocimum sanctum. African Journal
of Biotechnology, 20(5), 210–215.
https://doi.org/10.5897/ajb2020.17292
Alex, A. M., Subburaman, S., Chauhan, S., Ahuja, V., Abdi,
G., and Tarighat, M. A. (2024). Green synthesis of silver
nanoparticle prepared with Ocimum species and
assessment of anticancer potential. Scientific Reports,
14(1), 11707–11707.
https://doi.org/10.1038/s41598-024-61946-y
Anandalakshmi, K. (2021). Green synthesis of silver
nanoparticles using plant extracts – A review. Plant
Archives, 21, 2091–2097.
https://doi.org/10.51470/plantarchives.2021.v21.s1.345
Ansari, M., Ahmed, S., Abbasi, A., Khan, M. T., Subhan, M.,
Bukhari, N. A., Hatamleh, A. A., and Abdelsalam, N. R.
(2023). Plant mediated fabrication of silver
nanoparticles, process optimization, and impact on
tomato plant. Scientific Reports, 13(1), 18048–18048.
https://doi.org/10.1038/s41598-023-45038-x
Asgarpanah, J., and Mohajeri, M. (2012). “Phytochemistry
and medicinal properties of Ocimum sanctum L.”
Journal of Medicinal Plants Research, 6(15), 2892-2898.
Aynul, R., and Meyyappan, R. M. (2014). The antibacterial
effect of phyto-mediated silver nanoparticles produced
from Ocimum sanctum L. (Lamiaceae) leaf extract on
textile fabrics. African Journal of Microbiology
Research, 8(1), 118–128.
https://doi.org/10.5897/ajmr2013.6393
Banerjee, P., Satapathy, M. K., Mukhopadhayay, A., and
Das, P. (2014). Leaf extract mediated green synthesis of
silver nanoparticles from widely available Indian plants:
synthesis, characterization, antimicrobial property and
toxicity analysis. Bioresources and Bioprocessing, 1(1).
https://doi.org/10.1186/s40643-014-0003-y
Bansal, V., Rao, S. P., and Ghosh, A. (2012). “Green
synthesis of silver nanoparticles using Ocimum sanctum
(Holy Basil) extracts and their antibacterial activity.”
Applied Nanoscience, 2, 217-224.
doi:10.1007/s13204-012-0136-y.
Bhattacharya, D. and Gupta, R. (2005). “Nanotechnology
and the Environment.” Environmental Science and
Technology, 39(1), 480-486. DOI: 10.1021/es049137a.
Bindhani, B. K., and Panigrahi, A. K. (2015). Biosynthesis
and Characterization of Silver Nanoparticles (Snps) by
using Leaf Extracts of Ocimum Sanctum L (Tulsi) and
Study of its Antibacterial Activities. Journal of
Nanomedicine and Nanotechnology.
https://doi.org/10.4172/2157-7439.6.006
Bindhani, B., and Panigrahi, P. (2021). Synthesis and
characterization of silver nanoparticles using Ocimum
sanctum leaf extract. Journal of Nanobiotechnology,
19(1), 150. doi:10.1186/s12951-021-00920-y
Brahmachari, G., Sarkar, S., Ghosh, R., Barman, S., Mandal,
N. C., Jash, S. K., Banerjee, B., and Roy, R. (2014).
Sunlight-induced rapid and efficient biogenic synthesis
of silver nanoparticles using aqueous leaf extract of
Ocimum sanctum Linn. with enhanced antibacterial
activity. Organic and Medicinal Chemistry Letters, 4(1),
18–18. https://doi.org/10.1186/s13588-014-0018-6
Chaudhry, Q., and Castle, L. (2011). “Nanotechnology in
food production.” Nanotechnology in the Food Industry,
1, 125-136.
Das, U., Daimari, N. K., Biswas, R., and Mazumder, N.
(2024). Elucidating impact of solvent and pH in
synthesizing silver nanoparticles via green and chemical
route. Discover Applied Sciences, 6(6).
https://doi.org/10.1007/s42452-024-06010-0
Goyal, S. (2020). A Study on Synthesis of Silver
Nanoparticles Using Ocimum Sanctum L (Tulsi) Leaf
Extract and their Antimicrobial Activity: A Green
Chemistry Approach. Shodh International Journal of
Arts Science and Humanities, 8(1), 99–103.
https://doi.org/10.34293/sijash.v8i1.2450
Jain, D., et al. (2015). “Silver nanoparticles: a novel
nanomaterial to modulate DNA interactions.” Journal of
Nanoparticle Research, 17(8), 1-15. DOI:
10.1007/s11051-015-3076-3.
Jain, S., and Mehta, M. S. (2017). Medicinal Plant Leaf
Extract and Pure Flavonoid Mediated Green Synthesis of
Silver Nanoparticles and their Enhanced Antibacterial
Property. Scientific Reports, 7(1), 15867–15867.
https://doi.org/10.1038/s41598-017-15724-8
Khan, Md. Z. H., Tarek, F., Nuzat, M., Momin, Md. A., and
Hasan, Md. R. (2017). Rapid Biological Synthesis of
Silver Nanoparticles from Ocimum sanctum and Their
Characterization. Journal of Nanoscience, 2017, 1–6.
https://doi.org/10.1155/2017/1693416
Kumar, A., and Yadav, S. K. (2009). “Plant-mediated
synthesis of silver nanoparticles: a review.”
AJP-Advances in Plant Physiology, 134, 1-9.
Mallikarjuna, K., Golla, N., Dillip, G. R., Praveen, B.,
Shreedhar, B., Lakshmi, C. S., Reddy, B. V. S., and Raju,
B. D. P. (2011). Green synthesis of silver nanoparticles
using ocimum leaf extract and their characterization.
https://www.chalcogen.ro/181_Mallikarjuna.pdf
Manimaran, T., Sudhakar, T., Nanda, A., Bhat, M. A., and
Varghese, A. (2016). Biosynthesis of Green
Nanoparticles from Ocimum sanctum their
characterization. Research Journal of Pharmacy and
Technology, 9(4), 397–397.
https://doi.org/10.5958/0974-360X.2016.00072.x
nanoparticles using
the leaf extract of Ocimum sanctum and evaluation of their
antioxidant potential. Nano Express, 3(3), 35006–35006.
https://doi.org/10.1088/2632-959X/ ac8fac
Mishra, A., Kumar, S., and Singh, A. K. (2024). Biosynthesis
and characterization of Ocimum sanctum green silver
nanoparticles and unravelling their enhanced anti-filarial
activity through a HRAMS proteomics approach. RSC
Advances, 14(9), 5893–5906. https://doi.org/10.1039/
D3RA08702F
Mishra, S., Sundaram, S., Srivastava, S., and Dhar, R.
(2023). Phytosynthesis of Silver Nanoparticles Using
Ocimum sanctum Leaf Extract and Studies on Its
Antidiabetic, Antioxidant, and Antibacterial Properties.
ACS Applied Bio Materials, 6(10), 4127–4137.
https://doi.org/10.1021/acsabm.3c00234
Murdock, R.C., et al. (2008). “Characterization of
engineered nanoparticles.” Nature Nanotechnology,
3(5), 323-329. DOI: 10.1038/nnano.2008.187.
Narayanan, K. B., and Sakthivel, N. (2010). “Biological
synthesis of metal nanoparticles by microbes.” Advances
in Colloid and Interface Science, 156(1-2), 1-13.
doi:10.1016/j.cis.2010.01.002.
P.R., K. R., L., S., P.D. Gokulan, P. D. Gokulan, P., Devika,
P. Devika., P.M., E. P. M. E., and Jayaprithika.S,
Jayaprithika. S. (2025). Green Synthesis of Silver
Nanoparticles from Ocimum Sanctum leaf Extract and Its
Phytochemical Screening by Fourier Transform Infrared
Spectroscopy. International Journal of Pharmaceutical
Research and Applications, 10(1), 253–256.
https://doi.org/10.35629/4494-1001253256
Patel, R., and Kumar, S. (2022). Role of bioactive
compounds in the synthesis of silver nanoparticles from
plant extracts. Journal of Plant Sciences, 10(4), 330-340.
doi:10.11648/j.jps.20221004.12
Pathak, I., Niraula, M., Sharma, K. R., Thapa, P., Oli, H. B.,
and Kalauni, S. K. (2024). Green Synthesis of Silver
Nanoparticles from Ocimum sanctum Linn. and Study of
Their Antioxidant Activity. Amrit Research Journal,
5(1), 82–90. https://doi.org/10.3126/arj.v5i1.73553
Rajasiger, P., and Elangovan, K. (2018). “Preparation of
plant extracts for phytochemical analysis: a review.”
Journal of Pharmacognosy and Phytochemistry, 7(4),
3052-3055.
Ramdas, V. M., Botha, I., Krishna, S. B. N., and Mbatha, J.
N. (2015). Enhancing antidiabetic and antimicrobial
performance of Ocimum basilicum, and Ocimum
sanctum (L.) using silver nanoparticles. Saudi Journal of
Biological Sciences, 24(6), 1294–1305.
https://doi.org/10.1016/j.sjbs.2015.06.026
Ramteke, C., Chakrabarti, T., Sarangi, B. K., and Pandey,
R.-A. (2012). Synthesis of Silver Nanoparticles from the
Aqueous Extract of Leaves of Ocimum sanctum for
Enhanced Antibacterial Activity. Journal of Chemistry,
2013(1). https://doi.org/10.1155/2013/278925
Ranjan, P., and Gupta, D. K. (2015). “DNA interaction with
silver nanoparticles: Nanobiotechnology applications.”
International Journal of Nanomedicine, 10, 629-645.
doi:10.2147/IJN.S79980.
Ravi, K., and Chetan, E. (2022). Medicinal properties of
Ocimum sanctum: A review. Pharmacognosy Reviews,
16(32), 217-224. doi:10.5530/phrev.2022.16.32
Rout, Y. (2012). Green synthesis of silver nanoparticles
using Ocimum sanctum (Tulashi) and study of their
antibacterial and antifungal activities. Journal of
Microbiology and Antimicrobials, 4(6), 103–109.
https://doi.org/10.5897/JMA11.060
Sachin, P. Y., Vishnu Sharma, Devansh, Karan Dahiya,
Piyush., (2025). Phytochemical-Mediated Green
Synthesis of Silver Nanoparticles Using Eco-Extract of
Neem (Azadirachta indica) and Tulsi (Ocimum sanctum).
Zenodo (CERN European Organization for Nuclear
Research). https://doi.org/10.5281/zenodo.17811210
Sahu, N. G., and Padhy, R. N. (2013). “Synthesis of silver
nanoparticles from Ocimum sanctum leaf extract and its
antibacterial activity.” International Journal of Green
Chemistry, 1(3), 1-8. doi:10.6148/IJGC.2013.010302.
Sahu, R., and Khanna, P. (2023). Green synthesis of
nanoparticles: A comprehensive review on methods and
applications. Materials Today: Proceedings, 75,
1021-1032. doi:10.1016/j.matpr.2022.11.065
Sambrook, J., and Russell, D. W. (2001). Molecular
Cloning: A Laboratory Manual. 3rd Edition. Cold Spring
Harbor Laboratory Press, Cold Spring Harbor, NY.
Shahzadi, S., Fatima, S., Ain, Q. U., Shafiq, Z., and Janjua,
M. R. S. A. (2025). A review on green synthesis of silver
nanoparticles (SNPs) using plant extracts: a multifaceted
approach in photocatalysis, environmental remediation,
and biomedicine. RSC Advances, 15(5), 3858–3903.
https://doi.org/10.1039/d4ra07519f
Shankar, S. S., Rai, A., and Ankamwar, B. (2005).
“Biological synthesis of triangular silver nano-particles.”
Nature Nanotechnology, 4(6), 347-352. DOI:
10.1038/nnano.2009.157
Shankar, S. S., Rai, A., Poudel, B. K., and Tiwari, P. (2009).
“Biological synthesis of silver nanoparticles by the
fungus Neurospora crassa.” International Journal of
Nanotechnology, 6(7), 606-611.
doi:10.1504/IJNT.2009.023147.
Singh, K., Shukla, M. K., Singh, A. P., Pathak, J., Pandey, A.,
Sinha, R. P., and Singh, D. (2024). Biosynthesis and
biopotential assessment of silver nanoparticles (AGNPS)
by using leaf extract of Ocimum sanctum. International
Journal of Plant Pathology and Microbiology, 4(2),
34–41.
https://doi.org/10.22271/27893065.2024.v4.i2a.88
Sood, R., and Chopra, D. (2017). Improved yield of green
synthesized crystalline silver nanoparticles with potential
antioxidant activity. International Research Journal of
Pharmacy, 8(4), 100–104.
https://doi.org/10.7897/2230-8407.080457
Sulaiman, M., Muhammad, M. A., Sulaiman, A. S.,
Abubakar, A. L., Sharma, R., Shuaibu, A. M., Tiwari, R.,
and Mustapha, I. T. (2024). Antimicrobial potential and
characterization of silver nanoparticles synthesized from
Ocimum sanctum extract. GSC Biological and
Pharmaceutical Sciences, 28(1), 234–242.
https://doi.org/10.30574/gscbps.2024.28.1.1075
Tangod, A., and Namrata, K. (2020). Eco-friendly synthesis
of silver nanoparticles from Krishna Tulasi (Ocimum
sanctum) leaves. International Journal of Environmental
Science and Technology, 17(8), 3201-3210.
doi:10.1007/s13762-020-03012-8
Tangod, V. B., and M, N. (2024). Silver Nanoparticle
Synthesis using Ocimum Sanctum. International Journal
of Innovative Science and Modern Engineering, 12(2),
4–9. https://doi.org/10.35940/ijisme.F9560.12010124
Tessema, B., Tefera, G., and Bright, G. O. (2026b). Synthesis
of Silver Nanoparticles Using Reducing Agents of
Ocimum lamiifolium Leaves for the Application of
Anti-Bacterial Activity. Nanomaterials, 16(5), 283–283.
https://doi.org/10.3390/nano16050283
Tharwat, A., and El-Desoky, S. (2020). Reactive Oxygen
Species in Cancer: Mechanisms and Therapeutic
Strategies. Drug Development Research, 81(1), 105-115.
doi:10.1002/ddr.21538
Vahdati, R., and Sadeghi, N. (2019). DNA cleavage induced
by silver nanoparticles: Mechanisms and implications.
Biomaterials Science, 7(6), 2185-2194.
doi:10.1039/C8BM01085K
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